/usr/bin/varfilter.py is in samtools 1.7-1.
This file is owned by root:root, with mode 0o755.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 | #!/usr/bin/env python
#
# Copyright (C) 2009, 2010 Genome Research Ltd.
#
# Author: Aylwyn Scally <as6@sanger.ac.uk>
#
# Permission is hereby granted, free of charge, to any person obtaining a copy
# of this software and associated documentation files (the "Software"), to deal
# in the Software without restriction, including without limitation the rights
# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
# copies of the Software, and to permit persons to whom the Software is
# furnished to do so, subject to the following conditions:
#
# The above copyright notice and this permission notice shall be included in
# all copies or substantial portions of the Software.
#
# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL
# THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
# FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
# DEALINGS IN THE SOFTWARE.
# Author: lh3, converted to python and modified to add -C option by Aylwyn Scally
#
# About:
# varfilter.py is a port of Heng's samtools.pl varFilter script into
# python, with an additional -C INT option. This option sets a minimum
# consensus score, above which the script will output a pileup line
# wherever it _could have_ called a variant, even if none is actually
# called (i.e. hom-ref positions). This is important if you want to
# subsequently merge the calls with those for another individual to get a
# synoptic view of calls at each site. Without this option, and in all
# other respects, it behaves like samtools.pl varFilter.
#
# Aylwyn Scally as6@sanger.ac.uk
# Filtration code:
#
# C low CNS quality (hom-ref only)
# d low depth
# D high depth
# W too many SNPs in a window (SNP only)
# G close to a high-quality indel (SNP only)
# Q low RMS mapping quality (SNP only)
# g close to another indel with higher quality (indel only)
# s low SNP quality (SNP only)
# i low indel quality (indel only)
import sys
import getopt
def usage():
print '''usage: varfilter.py [options] [cns-pileup]
Options: -Q INT minimum RMS mapping quality for SNPs
-q INT minimum RMS mapping quality for gaps
-d INT minimum read depth
-D INT maximum read depth
-S INT minimum SNP quality
-i INT minimum indel quality
-C INT minimum consensus quality for hom-ref sites
-G INT min indel score for nearby SNP filtering
-w INT SNP within INT bp around a gap to be filtered
-W INT window size for filtering dense SNPs
-N INT max number of SNPs in a window
-l INT window size for filtering adjacent gaps
-p print filtered variants'''
def varFilter_aux(first, is_print):
try:
if first[1] == 0:
sys.stdout.write("\t".join(first[4:]) + "\n")
elif is_print:
sys.stderr.write("\t".join(["UQdDWGgsiCX"[first[1]]] + first[4:]) + "\n")
except IOError:
sys.exit()
mindepth = 3
maxdepth = 100
gapgapwin = 30
minsnpmapq = 25
mingapmapq = 10
minindelscore = 25
scorefactor = 100
snpgapwin = 10
densesnpwin = 10
densesnps = 2
printfilt = False
minsnpq = 0
minindelq = 0
mincnsq = 0
try:
options, args = getopt.gnu_getopt(sys.argv[1:], 'pq:d:D:l:Q:w:W:N:G:S:i:C:', [])
except getopt.GetoptError:
usage()
sys.exit(2)
for (oflag, oarg) in options:
if oflag == '-d': mindepth = int(oarg)
if oflag == '-D': maxdepth = int(oarg)
if oflag == '-l': gapgapwin = int(oarg)
if oflag == '-Q': minsnpmapq = int(oarg)
if oflag == '-q': mingapmapq = int(oarg)
if oflag == '-G': minindelscore = int(oarg)
if oflag == '-s': scorefactor = int(oarg)
if oflag == '-w': snpgapwin = int(oarg)
if oflag == '-W': densesnpwin = int(oarg)
if oflag == '-C': mincnsq = int(oarg)
if oflag == '-N': densesnps = int(oarg)
if oflag == '-p': printfilt = True
if oflag == '-S': minsnpq = int(oarg)
if oflag == '-i': minindelq = int(oarg)
if len(args) < 1:
inp = sys.stdin
else:
inp = open(args[0])
# calculate the window size
max_dist = max(gapgapwin, snpgapwin, densesnpwin)
staging = []
for t in (line.strip().split() for line in inp):
(flt, score) = (0, -1)
# non-var sites
if t[3] == '*/*':
continue
is_snp = t[2].upper() != t[3].upper()
if not (is_snp or mincnsq):
continue
# clear the out-of-range elements
while staging:
# Still on the same chromosome and the first element's window still affects this position?
if staging[0][4] == t[0] and int(staging[0][5]) + staging[0][2] + max_dist >= int(t[1]):
break
varFilter_aux(staging.pop(0), printfilt)
# first a simple filter
if int(t[7]) < mindepth:
flt = 2
elif int(t[7]) > maxdepth:
flt = 3
if t[2] == '*': # an indel
if minindelq and minindelq > int(t[5]):
flt = 8
elif is_snp:
if minsnpq and minsnpq> int(t[5]):
flt = 7
else:
if mincnsq and mincnsq > int(t[4]):
flt = 9
# site dependent filters
dlen = 0
if flt == 0:
if t[2] == '*': # an indel
# If deletion, remember the length of the deletion
(a,b) = t[3].split('/')
alen = len(a) - 1
blen = len(b) - 1
if alen>blen:
if a[0] == '-': dlen=alen
elif b[0] == '-': dlen=blen
if int(t[6]) < mingapmapq:
flt = 1
# filtering SNPs
if int(t[5]) >= minindelscore:
for x in (y for y in staging if y[3]):
# Is it a SNP and is it outside the SNP filter window?
if x[0] >= 0 or int(x[5]) + x[2] + snpgapwin < int(t[1]):
continue
if x[1] == 0:
x[1] = 5
# calculate the filtering score (different from indel quality)
score = int(t[5])
if t[8] != '*':
score += scorefactor * int(t[10])
if t[9] != '*':
score += scorefactor * int(t[11])
# check the staging list for indel filtering
for x in (y for y in staging if y[3]):
# Is it a SNP and is it outside the gap filter window
if x[0] < 0 or int(x[5]) + x[2] + gapgapwin < int(t[1]):
continue
if x[0] < score:
x[1] = 6
else:
flt = 6
break
else: # a SNP or hom-ref
if int(t[6]) < minsnpmapq:
flt = 1
# check adjacent SNPs
k = 1
for x in (y for y in staging if y[3]):
if x[0] < 0 and int(x[5]) + x[2] + densesnpwin >= int(t[1]) and (x[1] == 0 or x[1] == 4 or x[1] == 5):
k += 1
# filtering is necessary
if k > densesnps:
flt = 4
for x in (y for y in staging if y[3]):
if x[0] < 0 and int(x[5]) + x[2] + densesnpwin >= int(t[1]) and x[1] == 0:
x[1] = 4
else: # then check gap filter
for x in (y for y in staging if y[3]):
if x[0] < 0 or int(x[5]) + x[2] + snpgapwin < int(t[1]):
continue
if x[0] >= minindelscore:
flt = 5
break
staging.append([score, flt, dlen, is_snp] + t)
# output the last few elements in the staging list
while staging:
varFilter_aux(staging.pop(0), printfilt)
|